Abstract
Human lactoferrin (hLf) is an approximately 80 kDa iron-binding glycoprotein of the transferrin family. hLf is found in breast milk as one of the most important bioactive components with multiple functions, most notably antimicrobial and immunomodulatory activities. The objective of this work was to have an effective HPLC method applied for the quantitative analysis of hLf, besides of purification procedure to obtain lyophilized hLf from breast milk samples and its characterization, as an alternative for getting and monitoring a multifunctional protein. Lyophilized hLf was obtained by purification through a heparin affinity column followed by ultrafiltration with a 30 kDa membrane. The final solution was lyophilized and the product was analyzed by HPLC, FTIR spectroscopy, and SDS-PAGE electrophoresis. The product was stored at 4-8 °C and analyzed by HPLC after 1, 3, and 6 months to determine analytical stability. Lactoferrin purification was performed satisfactorily, obtaining an average recovery percentage of 90% of the protein after the column and 70% recovery of the molecule after ultrafiltration and lyophilization, demonstrating the presence of the protein by chromatographic, infrared, and electrophoretic analysis. After analytical stability analysis, the maximum protein loss was 11% after 6 months of storage. These results suggest that a lactoferrin product from breast milk with potential therapeutic functions is possible.
Presenters
Karina Jeanette Parra SaavedraProfesora, Farmacobiología, Universidad de Guadalajara. Centro Universitario de Ciencias Exactas e Ingenierías, Mexico Josue Raymundo Solís Pacheco
Profesor Investigador, Departamento de Farmacobiología, Universidad de Guadalajara, Jalisco, Mexico Adriana Macaria Macias Lamas
Profesor Investigador, Farmacobiologia, Universidad de Guadalajara, Mexico Blanca Rosa Aguilar Uscanga
Research Professor, Pharmacobiology, Centro Universitario de Ciencias Exactas e Ingenierías de la Universidad de Guadalajara, Jalisco, Mexico
Details
Presentation Type
Paper Presentation in a Themed Session
Theme
Interdisciplinary Health Sciences
KEYWORDS
Human lactoferrin, Lyophilized hLf, HPLC
