Abstract
Brewery spent grain (BSG) is an important residue from brewing industry, which is produced in large amounts (~155 tons per 1000 tons of beer produced). The BSG is mainly constituted by compounds of great interest recognized as renewable and abundant in the world (cellulose, hemicellulose and lignin). However, physical and chemical obstacles produced by lignocellulose recalcitrance due to the association of the components of lignocellulosic biomass (lignin-polysaccharide complex), hinder their efficient utilization. In consequence, the main challenge in biorefinery is the efficient fractionation and utilization of lignocellulosic biomass. In this way, the treatment with deep eutectic solvents (DES) is highlighted as a clean and efficient separation method, due to its ability to isolate hemicellulose and lignin fractions selectively, preserving the cellulose structure. This work presents an enrichment in polysaccharide content and improvement in enzymatic digestibility employing DES made up of choline chloride and lactic acid to enhance the BSG biorefinery by response surface methodology through the analysis of the operational conditions. The best operational condition obtained (1:8 mol/mol, 130°C and 90 min) generated a 75% enrichment of polysaccharide fraction and the remotion of 77.13%, 50.70% and 100% of acid soluble lignin, xylan, and arabinan respectively, which improved the saccharification near to 70% using Cellic CTec2 and 80% and 40% of glucan and xylan respectively using Aspergillus niger CECT 2700 enzymatic extract. Hence, this work provides a hopeful approach to fractionate the cellulose and lignin from BSG by DES pretreatment, which could be unfeasible to achieve using traditional pretreatment methods.
Presenters
José Manuel Domínguez GonzálezProfessor, Chemical Engineering, University of Vigo, Ourense, Spain María Guadalupe Morán Aguilar
Student, Doctorado, Universidad de Vigo, Ourense, Spain Iván Costa Trigo
Posdoctoral researcher, Chemical Engineering/BiotecnIA, University, Spain Alicia Paz
Postdoctoral researcher, Department of chemical engineering, Universidade de Vigo, Ourense, Spain Maria Guadalupe Aguilar Uscanga
Details
Presentation Type
Theme
Food Production and Sustainability
KEYWORDS
Biorefinery, Brewery Spent Grain, Deep Eutectic Solvent, Pretreatment, Cellulose Digestibility
