Thirteenth Global Studies Conference

Abstract

Lipases are enzymes that can be secreted by several microorganisms, making interesting the biodiversity exploration for searching new microorganisms able to produce these enzymes. Many agro-industrial residues can be used as potential substrates for production of enzymes. Lipolytic bacteria were isolated from oil contaminated soil and grown on tributyrin media containing 1 %( w/w) olive oil. The isolate activity was identified by Bergey`s manual. Rhodamine B agar medium was used to develop the culture medium for Lipase production. To check the optimization of media, various lipase activities were done (effect of carbon source, nitrogen source, temperature etc. on lipase). To check the presence of lipase Rhodamine B agar medium plates were prepared. On which lipase producing bacterial strain showed orange fluorescent colonies on Rhodamine B agar medium under U.V. light were isolated from oil contaminated soil. Lipase also finds use in health foods, chemicals and pharmaceuticals for transesterification and enantioselectivity. Recently, various strategies in the pharmaceutical and chemical industries have used lipases in the synthesis of optically pure drugs and agrochemicals that are more effective and produce relatively fewer side effects. The enantioselective and regioselective nature of lipases have been utilized for the resolution of chiral drugs, fat modification, synthesis of cocoa butter substituents, biofuels and for synthesis of personal care products, and flavor enhancers.