What is the Efficacy of iPSC and Gene-Editing Technology Inte ...

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  • Title: What is the Efficacy of iPSC and Gene-Editing Technology Intervention for Corrective Amyloid Accumulation in Familiar Early-Onset Alzheimer’s Disease?
  • Author(s): Martin Nwadiugwu
  • Publisher: Common Ground Research Networks
  • Collection: Common Ground Research Networks
  • Series: Technology, Knowledge & Society
  • Journal Title: The International Journal of Technology, Knowledge, and Society
  • Keywords: Amyloid Accumulation, Clustered Regularly Inter-Spaced Short Random Palindromic Repeats (CRISPR), Early-Onset Familiar Alzheimer’s Disease (EOFAD), Induced Pluripotent Stem Cell (iPSC)
  • Volume: 15
  • Issue: 4
  • Year: 2019
  • ISSN: 1832-3669 (Print)
  • DOI: https://doi.org/10.18848/1832-3669/CGP/v15i04/41-63
  • Citation: Nwadiugwu, Martin . 2019. "What is the Efficacy of iPSC and Gene-Editing Technology Intervention for Corrective Amyloid Accumulation in Familiar Early-Onset Alzheimer’s Disease?." The International Journal of Technology, Knowledge, and Society 15 (4): 41-63. doi:10.18848/1832-3669/CGP/v15i04/41-63.
  • Extent: 23 pages

Abstract

Stem cell (iPSCs) and CRISPR/Cas9 gene editing technology intervention for amyloid accumulation, and its potential to develop effective corrective strategies for dementia of Alzheimer’s type is the focus of this review. The search was conducted in three databases (MEDLINE, PubMed, and Scopus) according to the research question eligibility criteria employed using the PICO framework. The screening process yielded a total of thirty-four hits. Seven articles that met the eligibility criteria were included, critically appraised, and synthesized in the narration. Results showed that maximizing efficiency for induced stem-cell reprogramming depends on the purpose for its use, and that delivering high efficacy in disrupting mutant allele, and HDR specific knock-in of mutation using CRISPR has been more mixed. The review showed that iPSCs and CRISPR seem to achieve some level of performance for gene reprogramming and efficient introduction of gene mutation; while improved methods have been developed, iPSCs reprogramming is still limited and CRISPR provides only a limited efficient intervention for targeted gene-knock-in, therefore methods that improve targeting frequency and specificity should be investigated.